SANJIBAN MICROACTIVE


100% NATURAL ORGANIC LIQUID STIMULATOR ENHANCES BIOLOGICAL REMEDIATION

sanjibanlogo.gif (7752 bytes) THE GROWING NEED FOR BIOREMEDIATION
A major concern in the environment today is the contamination from waste dumps and effluent water systems. These above ground storage systems tend to develop thick surface crusts with heavy settling of solids at the bottom, commonly producing foul odours. The cause of these waste problems is simple. Unstable mixtures suffocate the natural biological activities in the pond. Healthy bacterial organisms that normally convert the waste into usable nutrients die from "overload". The solution to a healthy waste effluent system is to bring the natural microbial activity back into balance. "Bioremediation" is the application of biological treatment to the cleanup of unwanted contaminants. It incorporates the use of microorganisms such as bacteria and fungi to biodegrade contaminating products. Bioremediation is cost effective, environmentally sound, and increasingly the preferred choice of remedial technology for cleanup operations.

SANJIBAN MICROACTIVE
Sanjiban MicroActive is a liquid stimulator developed from a complex fermentation process. Easy-to-apply, non-toxic, completely safe and 100% natural, MicroActive is not a bacterial culture or an odour-masker, but an organic extract enriched with natural enzymes that activates and rapidly multiplies healthy organisms already present in the effluent system. As these microrganisms go to work to biodegrade the waste, the digestive process changes from anaerobic to aerobic, which quickly reduces offensive odours. Sanjiban MicroActive does not contain any live organisms or bacteria.

The Sanjiban Bio- remediation method is to "supercharge" the colonies of microbes that already exist in the effluent and are working to break down the waste materials. Sanjiban produces larger amounts of self-generating dissolved and free oxygen that inhibits odour-causing anaerobic activity. Properly applied, it is nature's own odour-neutralization process recreated and captured in a balanced micro-ecosystem. However, MicroActive will also stimulate and expand lab-cultured bacterial colonies which may be introduced into the waste ponds to enhance biological remediation. Along with reducing odours and breaking up crusts and solids, the biological activity stimulated by MicroActive also helps the waste retain more nutrient value.

There is a marked increase in organic matter as well as in macro and micronutrients. This is a direct result of a healthy and balanced microbial activity, with less of nutrient waste as a result of reduced loss in gases, settling and crusting. Often, the value of the retained nutrients more than compensates for MicroActive treatment. The essence of the Sanjiban MicroActive bio-remediation system is a synergistic action of insitu bio-augmentation and natural enzyme-based catalytic breakdown of organic wastes in effluent. This synergism will regenerate the natural eutrophic process in wastewaters. Sanjiban MicroActive aims to provide a natural solution to effluent treatment to enable the total return of wastewaters to life-supporting oxygenated water systems fit for farming and agriculture.

ADVANTAGES OF SANJIBAN MICROACTIVE TREATMENT

  • Will lower BOD/COD levels.
  • Will lower (TSS) Total Suspended Solids.
  • Will reduce (FOG) Fat/Oil/Grease levels.
  • Will increase MLSS levels.
  • Will eliminate waste odours.
  • Will increase water clarity.
  • Will enable water reuse for farming & cultivation.
  • Will reduce retention time & costs for effluent treatment.
  • Carton Packing:- 5 Litres x 4 Nos; 10 Litres x 2 Nos; 20 Litres x 1 No.

SANJIBAN MICROACTIVE IN SEWAGE TREATMENT
Sanjiban MicroActive is a liquid stimulator developed from a complex fermentation process. It is composed of natural extracts and enzymes that activate and rapidly multiply healthy microorganisms already present in the activated sludge in the sewage treatment plant. As these microorganisms go to work to biodegrade the pollutants in the effluent, the reduction in COD, BOD, nitrogen and phosphorous will increase rapidly. Sanjiban MicroActive produces large amounts of self-generating dissolved and free oxygen that help in reactivating the return sludge in sewage treatment plants.

ADVANTAGES OF USING SANJIBAN MICROACTIVE IN SEWAGE TREATMENT PLANT:
There are a number of advantages of using Sanjiban MicroActive in sewage treatment plants.
1.    Sanjiban MicroActive "supercharges" the colonies of microbes that already exist in activated sludge and increases the rate of breakdown of the organic waste, i.e. BOD & COD in sewage treatment.
2.    It improves the quality of biological floc and helps in producing more number of flocs. The type of sludge generated markedly increases its adsorptive properties.
3.    Sanjiban MicroActive encourages the growth of beneficial bacteria, smaller in size, and produces denser flocs. The dense flocs settle at a faster rate in the treated water and generate lesser volume of sludge.
4.    Sanjiban MicroActive discourages the growth of filamentous bacteria and reduces chances of bulking of sludge.
5.    Use of Sanjiban MicroActive continuously reactivates and stabilizes the returned sludge, as it produces large amounts of self-generating dissolved and free oxygen. Thus the activated sludge process is modified to "contact stabilization" i.e., process modification of activated sludge process to increase the capacity of treatment as compared to conventional plug flow. Use of Sanjiban MicroActive helps to modify existing conventional process at zero cost and increases the capacity of treatment.

Application Method and Dosage:
Sanjiban MicroActive is to be introduced into the "return sludge point" on Day 1 @ 2 Litres MicroActive concentrate per mld, (to be diluted 1:9 with water), as a shock dosage to kick start the remediation process. Repeat the same dosage on Day 4. Thereafter, from Day 7 onwards, maintenance dosage of 333 ml./mld of MicroActive is to be applied daily. Specific proprietary mix of microbial cultures blended with natural enzymes to further enhance bio-remediation is recommended.

SANJIBAN MICROACTIVE IN MANURE COMPOSTING PITS
Sanjiban MicroActive is enriched with natural enzymes that activate and rapidly multiply the healthy micro-organisms that already exist in the manure composting pit. As these micro- organisms go to work to biodegrade the waste, the digestive process in the compost pit changes from anaerobic to aerobic, which quickly reduces offensive odours and destroys pathogenic organisms. The synergistic action of bioaugmentation and enzymatic catalysis breaks down even the toughest of animal and plant wastes into a healthy, enriched, nutrient-balanced compost. Sanjiban MicroActive produces large amounts of self-generating dissolved and free oxygen, which accomplishes aeration without turning the compost pit either manually or mechanically, thus saving both labour and energy. The recycling of organic matter into humus, if not managed correctly, can limit fresh plant growth. Slow and incomplete organic matter recycling reduces nitrogen availability and increases the number of disease causing pathogens.

Sanjiban MicroActive helps in killing disease-causing organisms more efficiently during composting. The destruction is brought about by the combined action of high temperature (aerobic condition), exhaustion of nutrients (bioaugmentation of microbial population) as well as natural antibiosis. The massive microbial activity stimulated by Sanjiban MicroActive effectively carries out bio-remediation of purifying organic matter, thereby preventing flies and mosquitoes from breeding in the compost pit. The biological activity stimulated by Sanjiban MicroActive helps the compost retain more nutrient value and prevent losses due to leaching or escape of gases. There is a marked increase in organic matter as well as micronutrients. The beneficial soil bacteria and fungi produce gums and hyphae that glue and bind soil particles together to form improved aggregate. Complete composting frees up nitrogen for fresh growth and prevents negative bacteria production.

Sanjiban MicroActive, through a natural probiotic action, revitalises and rejuvenates the soil and plant system.

Application Method and Dosage
The most effective application method is to spray or sprinkle Sanjiban MicroActive layer by layer as the compost pit is built up. Alternatively, a number of vertical holes can be made in the manure pit and MicroActive poured in after dilution. Compost heaps should be covered with straw or plant-wastes to retain moisture. A normal dosage would be a single application of 100 ml.Sanjiban MicroActive per tonne of manure (diluted 1:9 with water to make a one litre solution).Composting should be complete in 30-45 days, dependent on ambient temperature and climatic conditions.

SANJIBAN MICROACTIVE FOR BIOREMEDIATION EUTROPHICATION OF NATRUAL WATER BODIES (LAGOONS)
A major concern in environment management is contamination from sewage and industrial effluent water of natural water bodies (lagoons). When sewage or industrial effluents feed the water bodies with pollutants and excessive phosphorus and nitrogen, there is over fertilization or eutrophication leading to algal bloom. This increased biomass may deplete the limited oxygen during the night, or when the sky is overcast for several days, and result in the death of the biomass in water bodies. Aquatic microorganisms are the natural work force to clean up water bodies when there is accidental input of pollutants. They carry out a bio-remediation action. Large-scale urbanization and industrialization has resulted in discharge of an immense amount of waste in natural water bodies.

This waste creates pollution in water bodies due to its low assimilative capacity. If a pre-positioned primary treatment unit can solve this problem, i.e., screen and grit chamber, then a constructed wetland system can be established to remove harmful organic compounds efficiently. However, applying Sanjiban MicroActive can directly increase the assimilative capacity of natural water bodies. Bio-remediation using Sanjiban MicroActive is cost effective, environmentally sound and the preferred choice of remedial technology for clean up operation of water bodies (lagoons).

ADVANTAGES OF USING SANJIBAN MICROACTIVE :
Sanjiban MicroActive is a liquid stimulator, enriched with natural enzymes that activates and rapidly multiplies healthy microorganisms in water bodies. As these microorganisms go to work to biodegrade the waste, the degradation process in the water bodies is accelerated, which quickly reduces pollutant loads. Sanjiban Bio-remediation method is to "supercharge" the colonies of microbes that already exist in water bodies and are working to breakdown the waste materials. Microactive produces large amounts of self-generating dissolved and free oxygen that increases dissolved oxygen throughout the water body upto the bottom layer. Properly applied, it accelerates the natural process of degradation and develops a balanced micro-ecosystem. MicroActive will also stimulate and expand lab-cultured bacterial colonies, which may be introduced into the waste ponds to enhance biological remediation.

ECOLOGICAL SYSTEMS (ECO-POND) FOR SUSTAINABLE MANAGEMENT:
Sanjiban MicroActive should be applied periodically for regular maintenance of the pond. Additionally, an ecological system formed through a multi-purpose utilization of the pond including growing aquatic plants, fish farming and duck/geese raising, which together with microorganisms, plankton and benthos constitute various food chains, and form a whole food web, can thus be established. In this system not only do bacteria and fungi act as decomposers, and algae and other aquatic plants as producers as in the conventional ponds, but also zooplankton, shrimp, clams and snails combine to constitute the ecopond system. In some well- designed and performing Eco-pond systems under warm climatic conditions, the typical removal efficiencies for various pollutants that have been achieved are: TSS 80-95%, BOD 85-98%, COD 80-93%, TKN 70-80%, NH4-N 85-95%, Phosphorus 90-95% and E coil bacteria 99%. Further, produce of 5000-6000 kg/ha of fish and 500-1000 kg/ha of shrimps can also be harvested.

APPLICATION METHOD AND DOSAGE:
To treat polluted water-bodies, an initial intensive shock treatment will usually be required. To accomplish this, Sanjiban MicroActive should first be sprayed over the treatment area at a rate of 10 litres of MicroActive concentrate (in a 1:9 dilution with water to make 100 litres solution) over an area 1000 sq.m. It is desirable to allow MicroActive to exist at the surface of the water- body as long as possible. After the surface treatment is completed, Sanjiban Microactive should be introduced evenly into the bottom layer of the pond at the same dosage rate of 10 litres MicroActive concentrate (to be diluted as above) in the same 1000 sq.m. area. With time, the eutrophic process will penetrate deeper, but initially, most of remedial action will be through surface layers, and therefore this deeper treatment is very effective.

Repeat this initial shock dosage again in 15 days, both on the surface as well as at the bottom of the pond. Thereafter, Sanjiban MicroActive should be applied once every 15 days, calculated at the rate of 20 litres concentrate (to be diluted as above) for every 10,000 sq.m. of water-body area. Application should preferably be at the influent ingress point, by continuous trickling method. Or else, the dosage can be sprayed over the pond water body. To enhance biological remediation, specific aquatic plants and fishes could also be introduced. (The total quantity of Sanjiban MicroActive required to treat a water body area of say 10,000 sq.m. will therefore be 200 litres for the initial shock treatment and thereafter 40 litres per month as a sustainable management dosage.)

SANJIBAN BIO-REMEDIATION IN BIOSALINE AGRICULTURE
A large percentage of mankind lives in coastal communities, within 50 kms. of the sea. Additionally, at certain periods of a year, there is a marked increase in population in these areas due to tourist inflow, creating added stress on environmental resources. Two-thirds of planet earth is covered by seawater, containing 3-5% of salts, mostly sodium chloride. Coastal communities are often forced to resort to brackish water irrigation to grow agricultural crops for their livelihood.

BIOSALINE AGRICULTURE
The success of saline irrigation in agriculture rests to a high degree on the management of the salt concentration in soil water. This is maintained by flushing the planted furrows periodically with seawater, much in simulation of the tidal inundation, which occurs in waves at the seashore. The optimal frequency of such inundation needs to be determined from site to site. It will differ depending on rainfall occurrence, frequency, seasonality, as well as the level of residual moisture in soil taking into account the local levels of evapotransportation. One of the relatively more successful methods employed in Biosaline Agriculture is the cyclic use of brackish water alternated with canal water irrigation.

Brackish water is used throughout the first season growing a salt-tolerant crop. The injurious effects do not curtail the yield of the first crop and the salt residues are washed out when the second crop is grown with canal water irrigation. The second crop needs to be a less salt-tolerant variety. This crop management strategy is cost-effective, practical and technically sound. Full crop water requirements are met, the cropping intensity is increased and the negative effects of brackish water are neutralised.

SANJIBAN IN BIOSALINE AGRICULTURE
Organic farming practices and manurial inputs can make a marked difference in Biosaline Agriculture. Application of farmyard manure, covering the soil with rice straw or other crop residues, growing green manure crops, introduction of haliophytic plants which will absorb the excess salts and heavy metals in the soil, are methods that will reduce salt stress in the soil, promote bacterial life and increase soil fertility. Sanjiban Bio-Active Composter is a natural organic biological stimulator derived through a complex biocatalyst fermentation process. Its unique mode of action activates and multiplies the beneficial waste-digesting microbes that are already present in the crop residues. Bio-Active Composter is also fortified with natural enzymes that successfully break down even the toughest of animal and plant wastes into a healthy, enriched, nutrient-balanced compost.

SANJIBAN BIO-ACTIVE COMPOSTER
Sanjiban Bio-Active Composter should be applied at the time of rice straw/crop residue covering. It will speedily decompose organic matter to create humus in the soil and will also dramatically increase the microbial life. Powerful natural enzymes in Bio-Active Composter decompose the crop residues into available food for microbial life and plants. (Application dosage 20 litres per acre diluted 1:9 with water).

SANJIBAN ROOT ZONE
Sanjiban Root Zone is a natural organic microbial growth stimulant that biologically activates the soil. This unique formulation is a liquid concentrate derived through a complex biocatalyst process. Its primary action is to multiply the soil's native microbes and bacteria and stimulate activity in the rhizosphere. This in turn breaks down available organic matter as well as releases inorganic elements bonded in the soil, resulting in a larger availability of organic acids, vitamins, growth regulators, enzymes and micro-nutrients. The large increase in microbial activity leads to a better-aerated soil, increased penetration of water and oxygen, and a reduction in compaction and soil alkanity.

The increase in oxygen at the molecular level coupled with better soil moisture retention, relieves stress and tends to balance soil pH. Sanjiban Root Zone should be applied to soil  once a month throughout the crop cycle, @ 20 litres concentrate per acre diluted 1:9 with water. To further enhance effective bio-remediation, scientific soil analysis reports will help determine specific custom-cultured natural microbial strains and specific natural enzymes which can then be innoculated into the soils to accelerate the remediation process. Sanjiban, through a probiotic action, helps populate beneficial micro-organisms in the soil to establish a healthy soil-plant microbiology. Sanjiban works in the soil so that the soil works better, naturally.

SANJIBAN MICROACTIVE BIO-REMEDIATION OF HYDROCARBON CONTAMINATION
Septic wastes from storage lagoons in petroleum refinery operations, tank-bottom sludges, and hydrocarbon-contaminated soils present serious difficulties in handling and disposal. Petroleum-derived waste is highly susceptible to contamination by anaerobic bacteria resulting in the release of noxious and volatile hydrogen sulphide and mercaptans. Bio-Remediation is a preferred means of cleanup for soils containing organic pollutants because it can provide a final solution through complete decomposition of the target compounds to acceptable regulatory levels. Soil and its complex microbial community can accept and degrade, to some degree, a variety of petroleum-based pollutants into non-toxic forms.

There exists in nature microbial forms (i.e. bacteria, fungi, yeast, molds, etc) which biodegrade many petroleum-based agents, which are noxious to the environment. These microbes produce extracellular enzymes, which lead to the breakdown, or mineralisation, of petroleum compounds, transforming them into food sources for use of the microbes in growth and reproduction. This process continues until all that remains is carbon dioxide, water, and biomass (fatty acids which are used as a good feed source by other microorganisms).

To date, Bio-Remediation has worked on the basis of microbes in the soil or water, which break down the long hydrocarbon molecular chains. Either specific lab-cultured microbes are introduced, or the indigenous bacterial colonies are provided nutrients and oxygen to increase their population, or both methods are employed.

The problem is that bacteria cannot easily digest the long hydrocarbon molecules. The molecules must penetrate two barriers – the cell wall of the bacteria, then the inner cytoplasmic membrane – prior to contacting the multi-enzyme complex within, and breaking down into easily digestible fatty acids. Normally, this process typically takes one to five years, depending on the complexity of the hydrocarbon It is these enzymatic compounds within the bacteria cell that actually oxidize the hydrocarbons.

THE SANJIBAN BIO-REMEDIATION PROCESS
Sanjiban MicroActive has a two-part action in the petroleum matrix, simultaneous and synergistic: on the one hand to create an aerobic environment by producing large quantities of dissolved oxygen at a molecular level to increase microbial population, and on the other hand breaking down the long hydrocarbon molecular chains to digestible fatty acids, carbon dioxide and water. MicroActive enzymes efficiently activate oxygen and convert it to a form in which the oxygen atom can be incorporated directly into the hydrocarbon compound, thus breaking the long- chain bonds. The hydrocarbon is then broken down to the corresponding alcohol, aldehyde, and finally to the monobasic fatty acids as this process repeats.

The indigenous bacterial population then readily digests these simple hydrocarbons. Once the reaction is completed, the enzyme breaks free to attach to another hydrocarbon source in order to repeat the same reaction. This is often referred to as the " lock and key" in that it is used many times. To further enhance and accelerate the bio-remediation process, specific lab-cultured bacteria customized to the specific hydrocarbon contaminant, may need be introduced into the waste degradation process, along with application of Sanjiban MicroActive. Additionally, SANJIBAN AQUAHUMUS, a balanced liquid microbial nutrient package, will enhance microbial effectivity. AquaHumus is a source of macronutrients, complexed with biotically digested organic complexes. It increases carbon and oxygen ratios by producing carbon and oxygen bonds in the soil and aqueous environments. Sanjiban AquaHumus also assists in microbial bonding to petroleum products, thus limiting volatilisation and enhancing degradation of petroleum hydrocarbons.

The Sanjiban Bio-Remediation Process uses a customized powerful enzymatic catalytic action to cleave the short-, long-, and branched-chain hydrocarbons and the hydrogen sulphide bonds of toxic compounds of hydrocarbon wastes, preventing their formation and eliminating odours. The remedial action effectively prevents volatilization of light-chain organic molecules such as benzene, toluene and xylenes and is also effective against recalcitrant compounds such as  polynuclear aromatics (creosote) and polychlorinated biphenyls (PCBs). Several unique properties of the Sanjiban Bio-Remediation Process enhance penetration into the soil and the petroleum matrix. MicroActive acts as a bio-surfactant making hydrocarbons more soluble and "digestible". Thus heavier long-chained hydrocarbons are cleaved into shorter- chain lighter fractions. This action reduces the surface tension and viscosity of most residues, including sludge, allowing easier handling capabilities.

As MicroActive is equally effective in acidic conditions, it breaks down the clay lattice in soils, diffusing rapidly through its pores, allowing the complex of enzymes easier access to the contaminants.

APPLICATION METHOD AND DOSAGE Pilot studies are required to be conducted to assess the precise dosage of Sanjiban MicroActive and AquaHumus needed in hydrocarbon contaminated sites. Additionally, specific microbial cultures, customised to the hydrocarbon contaminant will also be required to effectively accelerate the bio-remediation process.

Bio-venting to increase aeration at soil depths may need to be considered.

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